Mazinger Otome showcases the android versions of the mechas from the Mazinger franchise and Steel Jeeg series, as they integrate into everyday life. The story revolves around a group of female androids navigating high school and fending off the Mechanical Beast Army, along with school bullies. The lead character, Seto Magami, modeled after Mazinger Z, is regarded as Juzo Magami's granddaughter. The introduction of the New Mazinger Army leads to fierce confrontations and disagreements with the original androids.